Study sheds light on inflammation following an intracerebral hemorrhage

“The overall idea is to better understand the time course and mediators of inflammation after ICH so that the harmful inflammation can be inhibited and/or the beneficial inflammation can be promoted,” he said.

The researchers collected blood samples from 27 patients at less than 24 hours, around three days and around seven days after an ICH. 

“It's more challenging to collect blood early after ICH compared with later, but it was important for this study to have that first early blood draw in order to compare with the additional blood collections,” Walsh noted.

The blood samples were tested for messenger RNA (mRNA) — which shows how genes are functioning and being “expressed” in cells — and for microRNA (miRNA) — important regulatory molecules that modulate gene expression.

The team found neutrophils, the most abundant white blood cells in circulating blood, had the most significantly increased inflammatory processes at seven days following ICH compared to the first 24 hours. Walsh said it would be expected for inflammatory processes to peak in intensity around three days.

“Because we found this increased neutrophil signal at seven days compared with 72 hours, this suggests there could be something unique in ICH which causes more delayed neutrophil-driven inflammation,” he said. “If this is confirmed in larger studies, it could be a treatment target to modulate neutrophil function in ICH.” 

The miRNA analysis found miR-3613 and miR-3690 were most correlated with neutrophil-related genes.

“Because miRNA are such powerful regulators of gene expression, these miRNA could also be treatment targets if confirmed with additional research,” Walsh said. 

Walsh credited the multidisciplinary team of trial coordinators who screened and enrolled patients and collected and processed blood samples; experts in the UC Genomics, Epigenomics and Sequencing Core who performed the mRNA and miRNA sequencing; and bioinformatics collaborators who translated the raw data into meaningful results for helping to make the research successful.